Relationships among pathogenic and nonpathogenic isolates of Fusarium oxysporum based on the partial sequence of the intergenic spacer region of the ribosomal DNA.
Identifieur interne : 000290 ( Main/Exploration ); précédent : 000289; suivant : 000291Relationships among pathogenic and nonpathogenic isolates of Fusarium oxysporum based on the partial sequence of the intergenic spacer region of the ribosomal DNA.
Auteurs : D J Appel [États-Unis] ; T R GordonSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 1996.
Descripteurs français
- KwdFr :
- ADN ribosomique (composition chimique), ADN ribosomique (génétique), Amorces ADN (MeSH), Cartographie de restriction (MeSH), Deoxyribonuclease EcoRI (MeSH), Données de séquences moléculaires (MeSH), Fusarium (génétique), Fusarium (isolement et purification), Fusarium (pathogénicité), Introns (MeSH), Réaction de polymérisation en chaîne (MeSH), Similitude de séquences d'acides nucléiques (MeSH), Spécificité d'espèce (MeSH), Séquence conservée (MeSH), Séquence nucléotidique (MeSH), Virulence (génétique).
- MESH :
- composition chimique : ADN ribosomique.
- génétique : ADN ribosomique, Fusarium, Virulence.
- isolement et purification : Fusarium.
- pathogénicité : Fusarium.
- Amorces ADN, Cartographie de restriction, Deoxyribonuclease EcoRI, Données de séquences moléculaires, Introns, Réaction de polymérisation en chaîne, Similitude de séquences d'acides nucléiques, Spécificité d'espèce, Séquence conservée, Séquence nucléotidique.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Conserved Sequence (MeSH), DNA Primers (MeSH), DNA, Ribosomal (chemistry), DNA, Ribosomal (genetics), Deoxyribonuclease EcoRI (MeSH), Fusarium (genetics), Fusarium (isolation & purification), Fusarium (pathogenicity), Introns (MeSH), Molecular Sequence Data (MeSH), Polymerase Chain Reaction (MeSH), Restriction Mapping (MeSH), Sequence Homology, Nucleic Acid (MeSH), Species Specificity (MeSH), Virulence (genetics).
- MESH :
- chemical , chemistry : DNA, Ribosomal.
- chemical , genetics : DNA, Ribosomal.
- chemical : DNA Primers, Deoxyribonuclease EcoRI.
- genetics : Fusarium, Virulence.
- isolation & purification : Fusarium.
- pathogenicity : Fusarium.
- Base Sequence, Conserved Sequence, Introns, Molecular Sequence Data, Polymerase Chain Reaction, Restriction Mapping, Sequence Homology, Nucleic Acid, Species Specificity.
Abstract
Using PCR, we amplified and sequenced approximately 1,000 bp of the 5' end of the intergenic spacer (IGS) of the rDNA in 15 isolates of Fusarium oxysporum and one isolate of F. subglutinans. Isolates were selected to represent diversity in our collection based on differences in pathogenic race, vegetative compatibility group (VCG), mitochondrial DNA (mtDNA) haplotype, IGS haplotype, and DNA fingerprint. The objective of this research was to clarify the origin of virulence within F. oxysporum, the relationship between pathogenic and nonpathogenic strains, and the evolution of the different races of F. oxysporum f. sp. melonis. Bootstrapped parsimony analysis of the partial IGS sequence data identified a phylogenetic tree with highly significant branches. The two F. oxysporum f. sp. melonis VCGs, 0131 and 0134, were separated into distinct lineages. Race was not distinguished by significant IGS sequence differences within the pathogen VCGs. One exception was a race 1 isolate which was associated with VCG 0131 but, based on both mtDNA and IGS haplotype, had greater affinity with VCG 0134. Two IGS sequence types were found in this race 1 isolate, one suggesting an affiliation with VCG 0131 and the other similar to isolates in VCG 0134. This may have resulted from past somatic or sexual interactions between F. oxysporum f. sp. melonis, VCGs 0131 and 0134. Nonpathogens that were vegetatively compatible with the pathogen were not closely related to the pathogen based on IGS sequence data. Thus, nonpathogens and pathogens may share common alleles at vegetative compatibility loci by coincidence rather than because of recent clonal derivation from a common ancestor.
DOI: 10.1094/mpmi-9-0125
PubMed: 8820752
Affiliations:
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T R" uniqKey="Gordon T" first="T R" last="Gordon">T R Gordon</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1996">1996</date><idno type="RBID">pubmed:8820752</idno><idno type="pmid">8820752</idno><idno type="doi">10.1094/mpmi-9-0125</idno><idno type="wicri:Area/Main/Corpus">000291</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000291</idno><idno type="wicri:Area/Main/Curation">000291</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000291</idno><idno type="wicri:Area/Main/Exploration">000291</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Relationships among pathogenic and nonpathogenic isolates of Fusarium oxysporum based on the partial sequence of the intergenic spacer region of the ribosomal DNA.</title><author><name sortKey="Appel, D J" sort="Appel, D J" uniqKey="Appel D" first="D J" last="Appel">D J Appel</name><affiliation wicri:level="1"><nlm:affiliation>Department of Environmental Science, Policy, and Management, Division of Entomology, Plant and Soil Microbiology, University of California, Berkeley 94720, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Environmental Science, Policy, and Management, Division of Entomology, Plant and Soil Microbiology, University of California, Berkeley 94720</wicri:regionArea><placeName><settlement type="city">Berkeley (Californie)</settlement><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Gordon, T R" sort="Gordon, T R" uniqKey="Gordon T" first="T R" last="Gordon">T R Gordon</name></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>Conserved Sequence (MeSH)</term><term>DNA Primers (MeSH)</term><term>DNA, Ribosomal (chemistry)</term><term>DNA, Ribosomal (genetics)</term><term>Deoxyribonuclease EcoRI (MeSH)</term><term>Fusarium (genetics)</term><term>Fusarium (isolation & purification)</term><term>Fusarium (pathogenicity)</term><term>Introns (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Restriction Mapping (MeSH)</term><term>Sequence Homology, Nucleic Acid (MeSH)</term><term>Species Specificity (MeSH)</term><term>Virulence (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN ribosomique (composition chimique)</term><term>ADN ribosomique (génétique)</term><term>Amorces ADN (MeSH)</term><term>Cartographie de restriction (MeSH)</term><term>Deoxyribonuclease EcoRI (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Fusarium (génétique)</term><term>Fusarium (isolement et purification)</term><term>Fusarium (pathogénicité)</term><term>Introns (MeSH)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Similitude de séquences d'acides nucléiques (MeSH)</term><term>Spécificité d'espèce (MeSH)</term><term>Séquence conservée (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Virulence (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Ribosomal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Ribosomal</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term><term>Deoxyribonuclease EcoRI</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>ADN ribosomique</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fusarium</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN ribosomique</term><term>Fusarium</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Fusarium</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Fusarium</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Fusarium</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Fusarium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Conserved Sequence</term><term>Introns</term><term>Molecular Sequence Data</term><term>Polymerase Chain Reaction</term><term>Restriction Mapping</term><term>Sequence Homology, Nucleic Acid</term><term>Species Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Cartographie de restriction</term><term>Deoxyribonuclease EcoRI</term><term>Données de séquences moléculaires</term><term>Introns</term><term>Réaction de polymérisation en chaîne</term><term>Similitude de séquences d'acides nucléiques</term><term>Spécificité d'espèce</term><term>Séquence conservée</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Using PCR, we amplified and sequenced approximately 1,000 bp of the 5' end of the intergenic spacer (IGS) of the rDNA in 15 isolates of Fusarium oxysporum and one isolate of F. subglutinans. Isolates were selected to represent diversity in our collection based on differences in pathogenic race, vegetative compatibility group (VCG), mitochondrial DNA (mtDNA) haplotype, IGS haplotype, and DNA fingerprint. The objective of this research was to clarify the origin of virulence within F. oxysporum, the relationship between pathogenic and nonpathogenic strains, and the evolution of the different races of F. oxysporum f. sp. melonis. Bootstrapped parsimony analysis of the partial IGS sequence data identified a phylogenetic tree with highly significant branches. The two F. oxysporum f. sp. melonis VCGs, 0131 and 0134, were separated into distinct lineages. Race was not distinguished by significant IGS sequence differences within the pathogen VCGs. One exception was a race 1 isolate which was associated with VCG 0131 but, based on both mtDNA and IGS haplotype, had greater affinity with VCG 0134. Two IGS sequence types were found in this race 1 isolate, one suggesting an affiliation with VCG 0131 and the other similar to isolates in VCG 0134. This may have resulted from past somatic or sexual interactions between F. oxysporum f. sp. melonis, VCGs 0131 and 0134. Nonpathogens that were vegetatively compatible with the pathogen were not closely related to the pathogen based on IGS sequence data. Thus, nonpathogens and pathogens may share common alleles at vegetative compatibility loci by coincidence rather than because of recent clonal derivation from a common ancestor.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8820752</PMID><DateCompleted><Year>1996</Year><Month>12</Month><Day>18</Day></DateCompleted><DateRevised><Year>2019</Year><Month>11</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><Issue>2</Issue><PubDate><Year>1996</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Relationships among pathogenic and nonpathogenic isolates of Fusarium oxysporum based on the partial sequence of the intergenic spacer region of the ribosomal DNA.</ArticleTitle><Pagination><MedlinePgn>125-38</MedlinePgn></Pagination><Abstract><AbstractText>Using PCR, we amplified and sequenced approximately 1,000 bp of the 5' end of the intergenic spacer (IGS) of the rDNA in 15 isolates of Fusarium oxysporum and one isolate of F. subglutinans. Isolates were selected to represent diversity in our collection based on differences in pathogenic race, vegetative compatibility group (VCG), mitochondrial DNA (mtDNA) haplotype, IGS haplotype, and DNA fingerprint. The objective of this research was to clarify the origin of virulence within F. oxysporum, the relationship between pathogenic and nonpathogenic strains, and the evolution of the different races of F. oxysporum f. sp. melonis. Bootstrapped parsimony analysis of the partial IGS sequence data identified a phylogenetic tree with highly significant branches. The two F. oxysporum f. sp. melonis VCGs, 0131 and 0134, were separated into distinct lineages. Race was not distinguished by significant IGS sequence differences within the pathogen VCGs. One exception was a race 1 isolate which was associated with VCG 0131 but, based on both mtDNA and IGS haplotype, had greater affinity with VCG 0134. Two IGS sequence types were found in this race 1 isolate, one suggesting an affiliation with VCG 0131 and the other similar to isolates in VCG 0134. This may have resulted from past somatic or sexual interactions between F. oxysporum f. sp. melonis, VCGs 0131 and 0134. Nonpathogens that were vegetatively compatible with the pathogen were not closely related to the pathogen based on IGS sequence data. Thus, nonpathogens and pathogens may share common alleles at vegetative compatibility loci by coincidence rather than because of recent clonal derivation from a common ancestor.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Appel</LastName><ForeName>D J</ForeName><Initials>DJ</Initials><AffiliationInfo><Affiliation>Department of Environmental Science, Policy, and Management, Division of Entomology, Plant and Soil Microbiology, University of California, Berkeley 94720, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gordon</LastName><ForeName>T R</ForeName><Initials>TR</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004275">DNA, Ribosomal</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.1.21.-</RegistryNumber><NameOfSubstance UI="D015246">Deoxyribonuclease EcoRI</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017124" MajorTopicYN="N">Conserved Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004275" MajorTopicYN="N">DNA, Ribosomal</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015246" MajorTopicYN="N">Deoxyribonuclease EcoRI</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005670" MajorTopicYN="N">Fusarium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007438" MajorTopicYN="N">Introns</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015183" MajorTopicYN="N">Restriction Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012689" MajorTopicYN="N">Sequence Homology, Nucleic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1996</Year><Month>3</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1996</Year><Month>3</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1996</Year><Month>3</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8820752</ArticleId><ArticleId IdType="doi">10.1094/mpmi-9-0125</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region><settlement><li>Berkeley (Californie)</li></settlement></list><tree><noCountry><name sortKey="Gordon, T R" sort="Gordon, T R" uniqKey="Gordon T" first="T R" last="Gordon">T R Gordon</name></noCountry><country name="États-Unis"><region name="Californie"><name sortKey="Appel, D J" sort="Appel, D J" uniqKey="Appel D" first="D J" last="Appel">D J Appel</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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